p 4ebp1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc p 4ebp1
P 4ebp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+4ebp1/Phospho-4E-BP1+(Thr37%2F46)+Rabbit+mAb/us12594275-5287-38-39
Average 96 stars, based on 2472 article reviews

p 4ebp1 - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Immunophilin binding agents and uses thereof
Article Snippet: .. MW Antibody 1 Source Species Dilution Antibody 2 Source Species Dilution 100-55 P-AKT[S473] CST- Rabbit 1:1000 AKT CST- Mouse 1:1000 4060 2020 55-25 P-S6[S240/244] CST- Rabbit 1:2000 S6 CST- Mouse 1:1000 5364 2317 100+ P-ULK1[S757] Rabbit 1:1000 25− P-4EBP1[T37/46] CST- Rabbit 1:1000 FKBP12 Ab58072 Mouse 1:500 2855 55-25 GAPDH 60004- Mouse 1:50000 S6 CST- Rabbit 1:1000 1-Ig 2217 100+ ULK1 Rabbit 1:1000 25− 4EBP1 CST- Rabbit 1:1000 9644 Primary antibodies were added as solutions in 5% BSA-TBST, and the membranes were incubated at 4° C. overnight (16 h). ..

Formalin-fixed Paraffin-Embedded:

Article Title: Stimulator of interferon genes immunohistochemical expression in the spectrum of extrarenal perivascular epithelioid cell lesions.
Article Snippet: 269 270 Immunohistochemistry and fluorescence in situ hybridization (FISH) 271 Sections from tissue blocks of all tumors with available material, including four 272 metastases from two patients, as well as unstained sections for the remaining cases, were AR TIC LE IN PR ES S 10 273 immunohistochemically stained with STING (anti-TMEM173; clone SP338, dilution 1:150; 274 Abcam, UK). .. As for p-4EBP1 (clone 236B4, dilution 1:400; Cell Signaling), and p-S6K1 (clone 275 49D7, dilution 1:1000; Cell Signaling) testing, it was carried out in all cases with retrieved 276 FFPE blocks. .. Heat-induced antigen retrieval for STING was performed using a microwave 277 oven and 0.01mol/L of citrate buffer, pH 8.0, for 30 min. All samples were processed using a 278 sensitive ‘Bond Polymer Refine’ detection system in an automated Bond 279 immunohistochemistry instrument (Leica Biosystems, Germany).

Article Title: Stimulator of interferon genes immunohistochemical expression in the spectrum of extrarenal perivascular epithelioid cell lesions
Article Snippet: Sections from tissue blocks of all tumors with available material, including four metastases from two patients, as well as unstained sections for the remaining cases, were immunohistochemically stained with STING (anti-TMEM173; clone SP338, dilution 1:150; Abcam, UK). .. As for p-4EBP1 (clone 236B4, dilution 1:400; Cell Signaling), and p-S6K1 (clone 49D7, dilution 1:1000; Cell Signaling) testing, it was carried out in all cases with retrieved FFPE blocks. .. Heat-induced antigen retrieval for STING was performed using a microwave oven and 0.01 mol/L of citrate buffer, pH 8.0, for 30 min. All samples were processed using a sensitive ‘Bond Polymer Refine’ detection system in an automated Bond immunohistochemistry instrument (Leica Biosystems, Germany).

Cell Analysis:

Article Title: Active hemostatic constituents of Eclipta prostrata and PI3K-Akt-mediated protection in heat-induced gastric hemorrhage: Integration of serum-exposed metabolite profiling, network pharmacology, and in vivo validation.
Article Snippet: Eclipta prostrata, a traditional Chinese medicinal herb known as the “miracle hemostatic herb,” is clinically used for treating bleeding disorders.. However, the active components and mechanisms of action remain unclear.. This study aimed to reveal the active components and mechanisms of Eclipta prostrata in treating heat-induced gastric hemorrhage (HGH) through network pharmacology and experimental verification.

Western Blot:

Article Title: Enhanced Retinal Ganglion Cell Survival via Autophagy Activation in a Novel Retinal Ischemia/Reperfusion Rat Model
Article Snippet: 4EBP1 , Cell Signaling, 9644 , WB 1/1000. .. p-4EBP1 (Thr37/46) , Cell Signaling, 2855 , WB 1/1000. .. ULK1 , Cell Signaling, 8054 , WB 1/1000.

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